Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced

Ingrid Yamile Pulido Manrique; Carlos Alberto Jimenez Junca; Lina Marcela Mendez Castillo; Rosa Erlide Prieto Correa; Gilles Pieffet

doi:10.3390/ijms21113925

Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced

Ingrid Yamile Pulido Manrique (PHD Student)
, Carlos Alberto Jimenez Junca (Correspondent Author)
, Lina Marcela Mendez Castillo (masterstudent)
, Rosa Erlide Prieto Correa (Researcher)
, Gilles Pieffet (Third Author)

Universidad Antonio Nariño

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Translated title of the contribution	Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced
Original language	Undefined/Unknown
Pages (from-to)	1-21
Number of pages	20
Journal	International Journal of Molecular Sciences
DOIs	https://doi.org/10.3390/ijms21113925
State	Published - 29 May 2020

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10.3390/ijms21113925License: CC BY-ND

https://www.mdpi.com/1422-0067/21/11/3925License: CC BY-ND

Modificación Molecular De La Lipasa De Pseudomonas Aeruginosa Psa01, Para La Síntesis De Lípidos Estructurados (Opo) A Partir De Aceite De Palma.
Prieto Correa, R. E. (PI), Jimenez Junca, C. A. (PI), Pulido Manrique, I. Y. (PhDstudent), Quintanilla Carvajal, M. X. (Researcher) & Niñovásquez, L. F. (Asesor)
10/02/17 → 10/12/20
Project: Project Research

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Pulido Manrique, I. Y., Jimenez Junca, C. A., Mendez Castillo, L. M., Prieto Correa, R. E., & Pieffet, G. (2020). Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced. International Journal of Molecular Sciences, 1-21. https://doi.org/10.3390/ijms21113925

Pulido Manrique, Ingrid Yamile ; Jimenez Junca, Carlos Alberto ; Mendez Castillo, Lina Marcela et al. / Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced. In: International Journal of Molecular Sciences. 2020 ; pp. 1-21.

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title = "Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced",

author = "\{Pulido Manrique\}, \{Ingrid Yamile\} and \{Jimenez Junca\}, \{Carlos Alberto\} and \{Mendez Castillo\}, \{Lina Marcela\} and \{Prieto Correa\}, \{Rosa Erlide\} and Gilles Pieffet",

year = "2020",

month = may,

day = "29",

doi = "10.3390/ijms21113925",

language = "Indefinido/desconocido",

pages = "1--21",

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Pulido Manrique, IY, Jimenez Junca, CA, Mendez Castillo, LM, Prieto Correa, RE & Pieffet, G 2020, 'Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced', International Journal of Molecular Sciences, pp. 1-21. https://doi.org/10.3390/ijms21113925

Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced. / Pulido Manrique, Ingrid Yamile (PHD Student); Jimenez Junca, Carlos Alberto (Correspondent Author); Mendez Castillo, Lina Marcela (masterstudent) et al.
In: International Journal of Molecular Sciences, 29.05.2020, p. 1-21.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced

AU - Jimenez Junca, Carlos Alberto

AU - Pieffet, Gilles

A2 - Pulido Manrique, Ingrid Yamile

A2 - Mendez Castillo, Lina Marcela

A2 - Prieto Correa, Rosa Erlide

PY - 2020/5/29

Y1 - 2020/5/29

U2 - 10.3390/ijms21113925

DO - 10.3390/ijms21113925

M3 - Artículo

SN - 1422-0067

SP - 1

EP - 21

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

ER -

Pulido Manrique IY, Jimenez Junca CA, Mendez Castillo LM, Prieto Correa RE, Pieffet G. Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced. International Journal of Molecular Sciences. 2020 May 29;1-21. doi: 10.3390/ijms21113925

Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 ( DE3 ): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced

Article Classification

Indexación Internacional (Artículo)

Scopus-Q Quartil

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Projects

Modificación Molecular De La Lipasa De Pseudomonas Aeruginosa Psa01, Para La Síntesis De Lípidos Estructurados (Opo) A Partir De Aceite De Palma.

Cite this