Control of a Scenedesmus obliquus UTEX 393 Microalgae Culture Using Virtual Reference Feedback Tuning

Microalgae are photosynthetic microorganisms capable of fixing CO2 to produce O2 and a wide variety of metabolites of interest. Attempts have been made to describe their growth dynamics using mathematical models; however, these models fail to fully represent the dynamics of this bioprocess. Therefore, achieving maximum biomass production in the shortest possible time represents a control challenge due to the nonlinear and timevarying dynamics. Some classic control strategies implemented for this bioprocess are totally or partially dependent on a mathematical model, resulting in controllers with low performance, implementation complexity, and limited robustness. This is where the Virtual Reference Feedback Tuning (VRFT) approach becomes relevant, as it is a model-free control strategy. VRFT is based on the iterative generation of a virtual reference with the aim of minimizing steady-state error, without requiring an explicit model of the bioprocess. Its implementation involves the collection of experimental data in open loop, the minimization of a cost function in closed loop, and the linearization of the system around a stable equilibrium point. This work presents the design and implementation of a VRFT-based control strategy applied to the closed cultivation of the microalga Scenedesmus obliquus UTEX 393 in three flat photobioreactors at laboratory scale. The variables controlled using this strategy were temperature, photosynthetically active light intensity, and level. The experimental results showed that the pre-established references were met. A steady-state temperature of 25 ± 0.625 ◦C, a PAR (Photosynthetically Active Radiation) light intensity of 100 ± 5 μmol·m−2·s−1, and level control that ensured a constant volume of the culture medium were achieved. This suggests that VRFT is a viable control alternative for this type of bioprocess under nominal conditions.